Phenotypic and Genotypic Detection of Extended Spectrum β-lactamase and Carbapenemases Production Including bla TEM, bla PER and bla NDM-1 Genes Among Acinetobacter baumannii Clinical Isolates
نویسندگان
چکیده
منابع مشابه
detection of bla tem and bla shvgenes among clinical isolates of e. coli from tehran hospitals
background and objectives: the pathogenic strains of escherichia coli cause severe septicemia, urinary tract infection and wound sepsis. resistance of this organism to the expanded-spectrum cephalosporins occurs via the production of extended-spectrum β-lactamases (esbls) such as tem and shv. these enzymes hydrolyze oxyimino cephalosporins (cefotaxime, ceftazidime) and monobactams (aztreonam). ...
متن کاملSpread of bla(CTX-M-type) and bla(PER-2) beta-lactamase genes in clinical isolates from Bolivian hospitals.
OBJECTIVES To assess the prevalence and types of genes encoding extended-spectrum beta-lactamases (ESBLs) in clinical isolates of Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp. from Bolivia. METHODS A total of 642 clinical isolates were collected consecutively during a 4 month period (September to December 2004). Resistance or reduced susceptibility to cefotaxime and/or cef...
متن کاملPolyclonal spread of bla(OXA-23) and bla(OXA-58) in Acinetobacter baumannii isolates from Argentina.
BACKGROUND In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated from 1995 to 2006 with diminished susceptibilities to imipenem and meropenem. METHODOLOGY Acinetobacter baumannii isolates were identified with the ARDRA technique. The total g...
متن کاملbla(CTX-M-2) and bla(CTX-M-28) extended-spectrum beta-lactamase genes and class 1 integrons in clinical isolates of Klebsiella pneumoniae from Brazil.
Twenty-eight Klebsiella pneumoniae clinical isolates that exhibited an extended-spectrum cephalosporin-resistance profile from a city in the Northeast of Brazil were analysed by PCR and DNA sequencing in order to determine the occurrence of blaCTX-M genes and class 1 integrons. We determined the occurrence of the blaCTX-M-2 gene in six K. pneumoniae isolates and describe the first detection of ...
متن کاملMultiplex PCR for bla(CTX-M) & bla(SHV) in the extended spectrum beta lactamase (ESBL) producing gram-negative isolates.
BACKGROUND & OBJECTIVE Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in gram-negative isolates. ESBLs are mainly coded for by three important genes, namely bla(TEM), bla(SHV) and bla(CTX-M). In this study we used a multiplex PCR as a rapid method to identify two common genes (bla(CTX-M) & bla(SHV)) responsible for extended spectrum beta lactama...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2017
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.58206